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Polycomb directs timely activation of germline genes in spermatogenesis

During spermatogenesis, a large number of germline genes essential for male fertility are coordinately activated. However, it remains unknown how timely activation of this group of germline genes is accomplished. Here we show that Polycomb-repressive complex 1 (PRC1) directs timely activation of ger...

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Autores principales: Maezawa, So, Hasegawa, Kazuteru, Yukawa, Masashi, Sakashita, Akihiko, Alavattam, Kris G., Andreassen, Paul R., Vidal, Miguel, Koseki, Haruhiko, Barski, Artem, Namekawa, Satoshi H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647939/
https://www.ncbi.nlm.nih.gov/pubmed/28924034
http://dx.doi.org/10.1101/gad.302000.117
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author Maezawa, So
Hasegawa, Kazuteru
Yukawa, Masashi
Sakashita, Akihiko
Alavattam, Kris G.
Andreassen, Paul R.
Vidal, Miguel
Koseki, Haruhiko
Barski, Artem
Namekawa, Satoshi H.
author_facet Maezawa, So
Hasegawa, Kazuteru
Yukawa, Masashi
Sakashita, Akihiko
Alavattam, Kris G.
Andreassen, Paul R.
Vidal, Miguel
Koseki, Haruhiko
Barski, Artem
Namekawa, Satoshi H.
author_sort Maezawa, So
collection PubMed
description During spermatogenesis, a large number of germline genes essential for male fertility are coordinately activated. However, it remains unknown how timely activation of this group of germline genes is accomplished. Here we show that Polycomb-repressive complex 1 (PRC1) directs timely activation of germline genes during spermatogenesis. Inactivation of PRC1 in male germ cells results in the gradual loss of a stem cell population and severe differentiation defects, leading to male infertility. In the stem cell population, RNF2, the dominant catalytic subunit of PRC1, activates transcription of Sall4, which codes for a transcription factor essential for subsequent spermatogenic differentiation. Furthermore, RNF2 and SALL4 together occupy transcription start sites of germline genes in the stem cell population. Once differentiation commences, these germline genes are activated to enable the progression of spermatogenesis. Our study identifies a novel mechanism by which Polycomb directs the developmental process by activating a group of lineage-specific genes.
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spelling pubmed-56479392018-02-15 Polycomb directs timely activation of germline genes in spermatogenesis Maezawa, So Hasegawa, Kazuteru Yukawa, Masashi Sakashita, Akihiko Alavattam, Kris G. Andreassen, Paul R. Vidal, Miguel Koseki, Haruhiko Barski, Artem Namekawa, Satoshi H. Genes Dev Research Paper During spermatogenesis, a large number of germline genes essential for male fertility are coordinately activated. However, it remains unknown how timely activation of this group of germline genes is accomplished. Here we show that Polycomb-repressive complex 1 (PRC1) directs timely activation of germline genes during spermatogenesis. Inactivation of PRC1 in male germ cells results in the gradual loss of a stem cell population and severe differentiation defects, leading to male infertility. In the stem cell population, RNF2, the dominant catalytic subunit of PRC1, activates transcription of Sall4, which codes for a transcription factor essential for subsequent spermatogenic differentiation. Furthermore, RNF2 and SALL4 together occupy transcription start sites of germline genes in the stem cell population. Once differentiation commences, these germline genes are activated to enable the progression of spermatogenesis. Our study identifies a novel mechanism by which Polycomb directs the developmental process by activating a group of lineage-specific genes. Cold Spring Harbor Laboratory Press 2017-08-15 /pmc/articles/PMC5647939/ /pubmed/28924034 http://dx.doi.org/10.1101/gad.302000.117 Text en © 2017 Maezawa et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Paper
Maezawa, So
Hasegawa, Kazuteru
Yukawa, Masashi
Sakashita, Akihiko
Alavattam, Kris G.
Andreassen, Paul R.
Vidal, Miguel
Koseki, Haruhiko
Barski, Artem
Namekawa, Satoshi H.
Polycomb directs timely activation of germline genes in spermatogenesis
title Polycomb directs timely activation of germline genes in spermatogenesis
title_full Polycomb directs timely activation of germline genes in spermatogenesis
title_fullStr Polycomb directs timely activation of germline genes in spermatogenesis
title_full_unstemmed Polycomb directs timely activation of germline genes in spermatogenesis
title_short Polycomb directs timely activation of germline genes in spermatogenesis
title_sort polycomb directs timely activation of germline genes in spermatogenesis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5647939/
https://www.ncbi.nlm.nih.gov/pubmed/28924034
http://dx.doi.org/10.1101/gad.302000.117
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