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Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity

The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings and modeling of synaptic ac...

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Autores principales: Doussau, Frédéric, Schmidt, Hartmut, Dorgans, Kevin, Valera, Antoine M, Poulain, Bernard, Isope, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648531/
https://www.ncbi.nlm.nih.gov/pubmed/28990927
http://dx.doi.org/10.7554/eLife.28935
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author Doussau, Frédéric
Schmidt, Hartmut
Dorgans, Kevin
Valera, Antoine M
Poulain, Bernard
Isope, Philippe
author_facet Doussau, Frédéric
Schmidt, Hartmut
Dorgans, Kevin
Valera, Antoine M
Poulain, Bernard
Isope, Philippe
author_sort Doussau, Frédéric
collection PubMed
description The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings and modeling of synaptic activity at cerebellar granule cell to Purkinje cell synapses of mice, we describe two sub-pools in the RRP that can be differentially recruited upon ultrafast changes in the stimulation frequency. We show that at low-frequency stimulations, a first sub-pool is gradually silenced, leading to full blockage of synaptic transmission. Conversely, a second pool of synaptic vesicles that cannot be released by a single stimulus is recruited within milliseconds by high-frequency stimulation and support an ultrafast recovery of neurotransmitter release after low-frequency depression. This frequency-dependent mobilization or silencing of sub-pools in the RRP in terminals of granule cells may play a role in the filtering of sensorimotor information in the cerebellum.
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spelling pubmed-56485312017-10-23 Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity Doussau, Frédéric Schmidt, Hartmut Dorgans, Kevin Valera, Antoine M Poulain, Bernard Isope, Philippe eLife Neuroscience The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings and modeling of synaptic activity at cerebellar granule cell to Purkinje cell synapses of mice, we describe two sub-pools in the RRP that can be differentially recruited upon ultrafast changes in the stimulation frequency. We show that at low-frequency stimulations, a first sub-pool is gradually silenced, leading to full blockage of synaptic transmission. Conversely, a second pool of synaptic vesicles that cannot be released by a single stimulus is recruited within milliseconds by high-frequency stimulation and support an ultrafast recovery of neurotransmitter release after low-frequency depression. This frequency-dependent mobilization or silencing of sub-pools in the RRP in terminals of granule cells may play a role in the filtering of sensorimotor information in the cerebellum. eLife Sciences Publications, Ltd 2017-10-09 /pmc/articles/PMC5648531/ /pubmed/28990927 http://dx.doi.org/10.7554/eLife.28935 Text en © 2017, Doussau et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Doussau, Frédéric
Schmidt, Hartmut
Dorgans, Kevin
Valera, Antoine M
Poulain, Bernard
Isope, Philippe
Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity
title Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity
title_full Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity
title_fullStr Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity
title_full_unstemmed Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity
title_short Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity
title_sort frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648531/
https://www.ncbi.nlm.nih.gov/pubmed/28990927
http://dx.doi.org/10.7554/eLife.28935
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