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Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity
The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings and modeling of synaptic ac...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648531/ https://www.ncbi.nlm.nih.gov/pubmed/28990927 http://dx.doi.org/10.7554/eLife.28935 |
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author | Doussau, Frédéric Schmidt, Hartmut Dorgans, Kevin Valera, Antoine M Poulain, Bernard Isope, Philippe |
author_facet | Doussau, Frédéric Schmidt, Hartmut Dorgans, Kevin Valera, Antoine M Poulain, Bernard Isope, Philippe |
author_sort | Doussau, Frédéric |
collection | PubMed |
description | The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings and modeling of synaptic activity at cerebellar granule cell to Purkinje cell synapses of mice, we describe two sub-pools in the RRP that can be differentially recruited upon ultrafast changes in the stimulation frequency. We show that at low-frequency stimulations, a first sub-pool is gradually silenced, leading to full blockage of synaptic transmission. Conversely, a second pool of synaptic vesicles that cannot be released by a single stimulus is recruited within milliseconds by high-frequency stimulation and support an ultrafast recovery of neurotransmitter release after low-frequency depression. This frequency-dependent mobilization or silencing of sub-pools in the RRP in terminals of granule cells may play a role in the filtering of sensorimotor information in the cerebellum. |
format | Online Article Text |
id | pubmed-5648531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-56485312017-10-23 Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity Doussau, Frédéric Schmidt, Hartmut Dorgans, Kevin Valera, Antoine M Poulain, Bernard Isope, Philippe eLife Neuroscience The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings and modeling of synaptic activity at cerebellar granule cell to Purkinje cell synapses of mice, we describe two sub-pools in the RRP that can be differentially recruited upon ultrafast changes in the stimulation frequency. We show that at low-frequency stimulations, a first sub-pool is gradually silenced, leading to full blockage of synaptic transmission. Conversely, a second pool of synaptic vesicles that cannot be released by a single stimulus is recruited within milliseconds by high-frequency stimulation and support an ultrafast recovery of neurotransmitter release after low-frequency depression. This frequency-dependent mobilization or silencing of sub-pools in the RRP in terminals of granule cells may play a role in the filtering of sensorimotor information in the cerebellum. eLife Sciences Publications, Ltd 2017-10-09 /pmc/articles/PMC5648531/ /pubmed/28990927 http://dx.doi.org/10.7554/eLife.28935 Text en © 2017, Doussau et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Doussau, Frédéric Schmidt, Hartmut Dorgans, Kevin Valera, Antoine M Poulain, Bernard Isope, Philippe Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity |
title | Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity |
title_full | Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity |
title_fullStr | Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity |
title_full_unstemmed | Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity |
title_short | Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity |
title_sort | frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648531/ https://www.ncbi.nlm.nih.gov/pubmed/28990927 http://dx.doi.org/10.7554/eLife.28935 |
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