Calmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers

Store-operated calcium entry (SOCE) is a major pathway for calcium ions influx into cells and has a critical role in various cell functions. Here we demonstrate that calcium-bound calmodulin (Ca(2+)-CaM) binds to the core region of activated STIM1. This interaction facilitates slow Ca(2+)-dependent...

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Autores principales: Li, Xin, Wu, Guangyan, Yang, Yin, Fu, Shijuan, Liu, Xiaofen, Kang, Huimin, Yang, Xue, Su, Xun-Cheng, Shen, Yuequan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648805/
https://www.ncbi.nlm.nih.gov/pubmed/29051492
http://dx.doi.org/10.1038/s41467-017-01135-w
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author Li, Xin
Wu, Guangyan
Yang, Yin
Fu, Shijuan
Liu, Xiaofen
Kang, Huimin
Yang, Xue
Su, Xun-Cheng
Shen, Yuequan
author_facet Li, Xin
Wu, Guangyan
Yang, Yin
Fu, Shijuan
Liu, Xiaofen
Kang, Huimin
Yang, Xue
Su, Xun-Cheng
Shen, Yuequan
author_sort Li, Xin
collection PubMed
description Store-operated calcium entry (SOCE) is a major pathway for calcium ions influx into cells and has a critical role in various cell functions. Here we demonstrate that calcium-bound calmodulin (Ca(2+)-CaM) binds to the core region of activated STIM1. This interaction facilitates slow Ca(2+)-dependent inactivation after Orai1 channel activation by wild-type STIM1 or a constitutively active STIM1 mutant. We define the CaM-binding site in STIM1, which is adjacent to the STIM1–Orai1 coupling region. The binding of Ca(2+)-CaM to activated STIM1 disrupts the STIM1–Orai1 complex and also disassembles STIM1 oligomer. Based on these results we propose a model for the calcium-bound CaM-regulated deactivation of SOCE.
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spelling pubmed-56488052017-10-23 Calmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers Li, Xin Wu, Guangyan Yang, Yin Fu, Shijuan Liu, Xiaofen Kang, Huimin Yang, Xue Su, Xun-Cheng Shen, Yuequan Nat Commun Article Store-operated calcium entry (SOCE) is a major pathway for calcium ions influx into cells and has a critical role in various cell functions. Here we demonstrate that calcium-bound calmodulin (Ca(2+)-CaM) binds to the core region of activated STIM1. This interaction facilitates slow Ca(2+)-dependent inactivation after Orai1 channel activation by wild-type STIM1 or a constitutively active STIM1 mutant. We define the CaM-binding site in STIM1, which is adjacent to the STIM1–Orai1 coupling region. The binding of Ca(2+)-CaM to activated STIM1 disrupts the STIM1–Orai1 complex and also disassembles STIM1 oligomer. Based on these results we propose a model for the calcium-bound CaM-regulated deactivation of SOCE. Nature Publishing Group UK 2017-10-19 /pmc/articles/PMC5648805/ /pubmed/29051492 http://dx.doi.org/10.1038/s41467-017-01135-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Xin
Wu, Guangyan
Yang, Yin
Fu, Shijuan
Liu, Xiaofen
Kang, Huimin
Yang, Xue
Su, Xun-Cheng
Shen, Yuequan
Calmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers
title Calmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers
title_full Calmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers
title_fullStr Calmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers
title_full_unstemmed Calmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers
title_short Calmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers
title_sort calmodulin dissociates the stim1-orai1 complex and stim1 oligomers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648805/
https://www.ncbi.nlm.nih.gov/pubmed/29051492
http://dx.doi.org/10.1038/s41467-017-01135-w
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