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3D morphological analysis of the mouse cerebral vasculature: Comparison of in vivo and ex vivo methods
Ex vivo 2-photon fluorescence microscopy (2PFM) with optical clearing enables vascular imaging deep into tissue. However, optical clearing may also produce spherical aberrations if the objective lens is not index-matched to the clearing material, while the perfusion, clearing, and fixation procedure...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650181/ https://www.ncbi.nlm.nih.gov/pubmed/29053753 http://dx.doi.org/10.1371/journal.pone.0186676 |