Fundamental CRISPR-Cas9 tools and current applications in microbial systems

Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we outline the fundamental CRISPR tools that have been employed for strain optimization. These tools in...

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Detalles Bibliográficos
Autores principales: Tian, Pingfang, Wang, Jia, Shen, Xiaolin, Rey, Justin Forrest, Yuan, Qipeng, Yan, Yajun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655352/
https://www.ncbi.nlm.nih.gov/pubmed/29318202
http://dx.doi.org/10.1016/j.synbio.2017.08.006

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655352/
https://www.ncbi.nlm.nih.gov/pubmed/29318202
http://dx.doi.org/10.1016/j.synbio.2017.08.006

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