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Fundamental CRISPR-Cas9 tools and current applications in microbial systems
Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we outline the fundamental CRISPR tools that have been employed for strain optimization. These tools in...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
KeAi Publishing
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655352/ https://www.ncbi.nlm.nih.gov/pubmed/29318202 http://dx.doi.org/10.1016/j.synbio.2017.08.006 |