An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome

The λ-Red recombination system is a popular method for gene editing. However, its applications are limited due to restricted electroporation of DNA fragments. Here, we present an electroporation-free λ-Red recombination method in which target DNA fragments are excised by I-CreI endonuclease in vivo...

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Detalles Bibliográficos
Autores principales: Wei, Yanlong, Deng, Pingping, Mohsin, Ali, Yang, Yan, Zhou, Huayan, Guo, Meijin, Fang, Hongqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655456/
https://www.ncbi.nlm.nih.gov/pubmed/29065183
http://dx.doi.org/10.1371/journal.pone.0186891

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655456/
https://www.ncbi.nlm.nih.gov/pubmed/29065183
http://dx.doi.org/10.1371/journal.pone.0186891

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