Multiplexed genetic engineering of human hematopoietic stem and progenitor cells using CRISPR/Cas9 and AAV6

Precise and efficient manipulation of genes is crucial for understanding the molecular mechanisms that govern human hematopoiesis and for developing novel therapies for diseases of the blood and immune system. Current methods do not enable precise engineering of complex genotypes that can be easily...

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Detalles Bibliográficos
Autores principales: Bak, Rasmus O, Dever, Daniel P, Reinisch, Andreas, Cruz Hernandez, David, Majeti, Ravindra, Porteus, Matthew H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Stem Cells and Regenerative Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656432/
https://www.ncbi.nlm.nih.gov/pubmed/28956530
http://dx.doi.org/10.7554/eLife.27873

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656432/
https://www.ncbi.nlm.nih.gov/pubmed/28956530
http://dx.doi.org/10.7554/eLife.27873

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