Ecobody technology: rapid monoclonal antibody screening method from single B cells using cell-free protein synthesis for antigen-binding fragment formation

We report a rapid and cost-effective monoclonal antibody screening method from single animal B cells using reverse transcription (RT)-PCR and Escherichia coli cell-free protein synthesis (CFPS), which allows evaluation of antibodies within 2 working days. This process is named “Ecobody technology”....

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Detalles Bibliográficos
Autores principales: Ojima-Kato, Teruyo, Nagai, Satomi, Nakano, Hideo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656612/
https://www.ncbi.nlm.nih.gov/pubmed/29070795
http://dx.doi.org/10.1038/s41598-017-14277-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656612/
https://www.ncbi.nlm.nih.gov/pubmed/29070795
http://dx.doi.org/10.1038/s41598-017-14277-0

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