A Lactococcus lactis expression vector set with multiple affinity tags to facilitate isolation and direct labeling of heterologous secreted proteins

The gram-positive bacterium Lactococcus lactis is a useful host for extracellular protein production. A main advantage of L. lactis over other bacterial expression systems is that lactococcal cells display low levels of autolysis and proteolysis. Previously, we developed a set of vectors for nisin-i...

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Detalles Bibliográficos
Autores principales: Romero Pastrana, Francisco, Neef, Jolanda, van Dijl, Jan Maarten, Buist, Girbe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Applied Genetics and Molecular Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656699/
https://www.ncbi.nlm.nih.gov/pubmed/28971274
http://dx.doi.org/10.1007/s00253-017-8524-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656699/
https://www.ncbi.nlm.nih.gov/pubmed/28971274
http://dx.doi.org/10.1007/s00253-017-8524-x

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