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A Lactococcus lactis expression vector set with multiple affinity tags to facilitate isolation and direct labeling of heterologous secreted proteins
The gram-positive bacterium Lactococcus lactis is a useful host for extracellular protein production. A main advantage of L. lactis over other bacterial expression systems is that lactococcal cells display low levels of autolysis and proteolysis. Previously, we developed a set of vectors for nisin-i...
Autores principales: | Romero Pastrana, Francisco, Neef, Jolanda, van Dijl, Jan Maarten, Buist, Girbe |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656699/ https://www.ncbi.nlm.nih.gov/pubmed/28971274 http://dx.doi.org/10.1007/s00253-017-8524-x |
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