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Evolved α‐factor prepro‐leaders for directed laccase evolution in Saccharomyces cerevisiae
Although the functional expression of fungal laccases in Saccharomyces cerevisiae has proven to be complicated, the replacement of signal peptides appears to be a suitable approach to enhance secretion in directed evolution experiments. In this study, twelve constructs were prepared by fusing native...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658585/ https://www.ncbi.nlm.nih.gov/pubmed/28805314 http://dx.doi.org/10.1111/1751-7915.12838 |