CRISPR/Cas9 screening using unique molecular identifiers

Loss‐of‐function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the guide library, which act as...

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Detalles Bibliográficos
Autores principales: Schmierer, Bernhard, Botla, Sandeep K, Zhang, Jilin, Turunen, Mikko, Kivioja, Teemu, Taipale, Jussi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658704/
https://www.ncbi.nlm.nih.gov/pubmed/28993443
http://dx.doi.org/10.15252/msb.20177834

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658704/
https://www.ncbi.nlm.nih.gov/pubmed/28993443
http://dx.doi.org/10.15252/msb.20177834

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