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CRISPR/Cas9 screening using unique molecular identifiers

Loss‐of‐function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the guide library, which act as...

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Detalles Bibliográficos
Autores principales:	Schmierer, Bernhard, Botla, Sandeep K, Zhang, Jilin, Turunen, Mikko, Kivioja, Teemu, Taipale, Jussi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2017
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658704/ https://www.ncbi.nlm.nih.gov/pubmed/28993443 http://dx.doi.org/10.15252/msb.20177834

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