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A method to increase reproducibility in adult ventricular myocyte sizing and flow cytometry: Avoiding cell size bias in single cell preparations

RATIONALE: Flow cytometry (FCM) of ventricular myocytes (VMs) is an emerging technology in adult cardiac research that is challenged by the wide variety of VM shapes and sizes. Cellular variability and cytometer flow cell size can affect cytometer performance. These two factors of variance limit ass...

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Detalles Bibliográficos
Autores principales: López, Javier E., Jaradeh, Katrin, Silva, Emmanuel, Aminololama-Shakeri, Shadi, Simpson, Paul C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5662089/
https://www.ncbi.nlm.nih.gov/pubmed/29084228
http://dx.doi.org/10.1371/journal.pone.0186792