Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors

The nuclease-deactivated variant of CRISPR-Cas9 proteins (dCas9) fused to heterologous transactivation domains can act as a potent guide RNA sequence-directed inducer or repressor of gene expression in mammalian cells. In such a system the long-term presence of a stable dCas9 effector can be a draw-...

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Detalles Bibliográficos
Autores principales: Kleinjan, Dirk A., Wardrope, Caroline, Nga Sou, Si, Rosser, Susan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5662744/
https://www.ncbi.nlm.nih.gov/pubmed/29084946
http://dx.doi.org/10.1038/s41467-017-01222-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5662744/
https://www.ncbi.nlm.nih.gov/pubmed/29084946
http://dx.doi.org/10.1038/s41467-017-01222-y

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