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Utilizing Biotinylated Proteins Expressed in Yeast to Visualize DNA–Protein Interactions at the Single-Molecule Level

Much of our knowledge in conventional biochemistry has derived from bulk assays. However, many stochastic processes and transient intermediates are hidden when averaged over the ensemble. The powerful technique of single-molecule fluorescence microscopy has made great contributions to the understand...

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Detalles Bibliográficos
Autores principales: Xue, Huijun, Bei, Yuanyuan, Zhan, Zhengyan, Chen, Xiuqiang, Xu, Xin, Fu, Yu V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5662892/
https://www.ncbi.nlm.nih.gov/pubmed/29123507
http://dx.doi.org/10.3389/fmicb.2017.02062