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The influence of microRNAs and poly(A) tail length on endogenous mRNA–protein complexes
BACKGROUND: All mRNAs are bound in vivo by proteins to form mRNA–protein complexes (mRNPs), but changes in the composition of mRNPs during posttranscriptional regulation remain largely unexplored. Here, we have analyzed, on a transcriptome-wide scale, how microRNA-mediated repression modulates the a...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664449/ https://www.ncbi.nlm.nih.gov/pubmed/29089021 http://dx.doi.org/10.1186/s13059-017-1330-z |
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author | Rissland, Olivia S. Subtelny, Alexander O. Wang, Miranda Lugowski, Andrew Nicholson, Beth Laver, John D. Sidhu, Sachdev S. Smibert, Craig A. Lipshitz, Howard D. Bartel, David P. |
author_facet | Rissland, Olivia S. Subtelny, Alexander O. Wang, Miranda Lugowski, Andrew Nicholson, Beth Laver, John D. Sidhu, Sachdev S. Smibert, Craig A. Lipshitz, Howard D. Bartel, David P. |
author_sort | Rissland, Olivia S. |
collection | PubMed |
description | BACKGROUND: All mRNAs are bound in vivo by proteins to form mRNA–protein complexes (mRNPs), but changes in the composition of mRNPs during posttranscriptional regulation remain largely unexplored. Here, we have analyzed, on a transcriptome-wide scale, how microRNA-mediated repression modulates the associations of the core mRNP components eIF4E, eIF4G, and PABP and of the decay factor DDX6 in human cells. RESULTS: Despite the transient nature of repressed intermediates, we detect significant changes in mRNP composition, marked by dissociation of eIF4G and PABP, and by recruitment of DDX6. Furthermore, although poly(A)-tail length has been considered critical in post-transcriptional regulation, differences in steady-state tail length explain little of the variation in either PABP association or mRNP organization more generally. Instead, relative occupancy of core components correlates best with gene expression. CONCLUSIONS: These results indicate that posttranscriptional regulatory factors, such as microRNAs, influence the associations of PABP and other core factors, and do so without substantially affecting steady-state tail length. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13059-017-1330-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5664449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-56644492017-11-08 The influence of microRNAs and poly(A) tail length on endogenous mRNA–protein complexes Rissland, Olivia S. Subtelny, Alexander O. Wang, Miranda Lugowski, Andrew Nicholson, Beth Laver, John D. Sidhu, Sachdev S. Smibert, Craig A. Lipshitz, Howard D. Bartel, David P. Genome Biol Research BACKGROUND: All mRNAs are bound in vivo by proteins to form mRNA–protein complexes (mRNPs), but changes in the composition of mRNPs during posttranscriptional regulation remain largely unexplored. Here, we have analyzed, on a transcriptome-wide scale, how microRNA-mediated repression modulates the associations of the core mRNP components eIF4E, eIF4G, and PABP and of the decay factor DDX6 in human cells. RESULTS: Despite the transient nature of repressed intermediates, we detect significant changes in mRNP composition, marked by dissociation of eIF4G and PABP, and by recruitment of DDX6. Furthermore, although poly(A)-tail length has been considered critical in post-transcriptional regulation, differences in steady-state tail length explain little of the variation in either PABP association or mRNP organization more generally. Instead, relative occupancy of core components correlates best with gene expression. CONCLUSIONS: These results indicate that posttranscriptional regulatory factors, such as microRNAs, influence the associations of PABP and other core factors, and do so without substantially affecting steady-state tail length. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13059-017-1330-z) contains supplementary material, which is available to authorized users. BioMed Central 2017-10-31 /pmc/articles/PMC5664449/ /pubmed/29089021 http://dx.doi.org/10.1186/s13059-017-1330-z Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Rissland, Olivia S. Subtelny, Alexander O. Wang, Miranda Lugowski, Andrew Nicholson, Beth Laver, John D. Sidhu, Sachdev S. Smibert, Craig A. Lipshitz, Howard D. Bartel, David P. The influence of microRNAs and poly(A) tail length on endogenous mRNA–protein complexes |
title | The influence of microRNAs and poly(A) tail length on endogenous mRNA–protein complexes |
title_full | The influence of microRNAs and poly(A) tail length on endogenous mRNA–protein complexes |
title_fullStr | The influence of microRNAs and poly(A) tail length on endogenous mRNA–protein complexes |
title_full_unstemmed | The influence of microRNAs and poly(A) tail length on endogenous mRNA–protein complexes |
title_short | The influence of microRNAs and poly(A) tail length on endogenous mRNA–protein complexes |
title_sort | influence of micrornas and poly(a) tail length on endogenous mrna–protein complexes |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664449/ https://www.ncbi.nlm.nih.gov/pubmed/29089021 http://dx.doi.org/10.1186/s13059-017-1330-z |
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