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Study of in vitro transcriptional binding effects and noise using constitutive promoters combined with UP element sequences in Escherichia coli
BACKGROUND: UP elements (upstream element) are DNA sequences upstream of a promoter that interact with the α-subunit of RNA polymerase (RNAP) and can affect transcription by altering the binding RNAP to DNA. However, details of UP element and binding affinity effects on transcriptional strength are...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664571/ https://www.ncbi.nlm.nih.gov/pubmed/29118850 http://dx.doi.org/10.1186/s13036-017-0075-2 |