TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression

During the last few decades, the recombinant protein expression finds more and more applications. The cloning of protein-coding genes into expression vectors is required to be directional for proper expression, and versatile in order to facilitate gene insertion in multiple different vectors for exp...

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Detalles Bibliográficos
Autores principales: Niarchos, Athanasios, Siora, Anastasia, Konstantinou, Evangelia, Kalampoki, Vasiliki, Lagoumintzis, George, Poulas, Konstantinos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665511/
https://www.ncbi.nlm.nih.gov/pubmed/29091919
http://dx.doi.org/10.1371/journal.pone.0186568

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665511/
https://www.ncbi.nlm.nih.gov/pubmed/29091919
http://dx.doi.org/10.1371/journal.pone.0186568

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