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TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression
During the last few decades, the recombinant protein expression finds more and more applications. The cloning of protein-coding genes into expression vectors is required to be directional for proper expression, and versatile in order to facilitate gene insertion in multiple different vectors for exp...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665511/ https://www.ncbi.nlm.nih.gov/pubmed/29091919 http://dx.doi.org/10.1371/journal.pone.0186568 |