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Sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions
Technologies to sequence nucleic acids/proteins are widely available, but straightforward methodologies to sequence complex polysaccharides are lacking. We here put forward a strategy to sequence glycosaminoglycans, long linear polysaccharides involved in many biochemical processes. The method is ba...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665995/ https://www.ncbi.nlm.nih.gov/pubmed/29093576 http://dx.doi.org/10.1038/s41598-017-15009-0 |
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author | van Kuppevelt, Toin H. Oosterhof, Arie Versteeg, Elly M. M. Podhumljak, Emina van de Westerlo, Els M. A. Daamen, Willeke F. |
author_facet | van Kuppevelt, Toin H. Oosterhof, Arie Versteeg, Elly M. M. Podhumljak, Emina van de Westerlo, Els M. A. Daamen, Willeke F. |
author_sort | van Kuppevelt, Toin H. |
collection | PubMed |
description | Technologies to sequence nucleic acids/proteins are widely available, but straightforward methodologies to sequence complex polysaccharides are lacking. We here put forward a strategy to sequence glycosaminoglycans, long linear polysaccharides involved in many biochemical processes. The method is based on the covalent immobilization and (immuno)chemical characterization of only those size-separated saccharides that harbor the original reducing end of the full-length chain. Using this methodology, the saccharide sequence of the chondroitin sulfate chain of the proteoglycan bikunin was determined. The method can be performed in any standard biochemical lab and opens studies to the interaction of complex saccharide sequences with other biomolecules. |
format | Online Article Text |
id | pubmed-5665995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56659952017-11-08 Sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions van Kuppevelt, Toin H. Oosterhof, Arie Versteeg, Elly M. M. Podhumljak, Emina van de Westerlo, Els M. A. Daamen, Willeke F. Sci Rep Article Technologies to sequence nucleic acids/proteins are widely available, but straightforward methodologies to sequence complex polysaccharides are lacking. We here put forward a strategy to sequence glycosaminoglycans, long linear polysaccharides involved in many biochemical processes. The method is based on the covalent immobilization and (immuno)chemical characterization of only those size-separated saccharides that harbor the original reducing end of the full-length chain. Using this methodology, the saccharide sequence of the chondroitin sulfate chain of the proteoglycan bikunin was determined. The method can be performed in any standard biochemical lab and opens studies to the interaction of complex saccharide sequences with other biomolecules. Nature Publishing Group UK 2017-11-01 /pmc/articles/PMC5665995/ /pubmed/29093576 http://dx.doi.org/10.1038/s41598-017-15009-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article van Kuppevelt, Toin H. Oosterhof, Arie Versteeg, Elly M. M. Podhumljak, Emina van de Westerlo, Els M. A. Daamen, Willeke F. Sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions |
title | Sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions |
title_full | Sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions |
title_fullStr | Sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions |
title_full_unstemmed | Sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions |
title_short | Sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions |
title_sort | sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665995/ https://www.ncbi.nlm.nih.gov/pubmed/29093576 http://dx.doi.org/10.1038/s41598-017-15009-0 |
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