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Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions

BACKGROUND: Recombinant protein production and purification from Escherichia coli is often accompanied with expensive and complicated procedures, especially for therapeutic proteins. Here it was demonstrated that, by using an intein cleavable polyhydroxyalkanoate synthase fusion, recombinant protein...

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Detalles Bibliográficos
Autores principales: Du, Jinping, Rehm, Bernd H. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5667439/
https://www.ncbi.nlm.nih.gov/pubmed/29096637
http://dx.doi.org/10.1186/s12934-017-0799-1