Cargando…
Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions
BACKGROUND: Recombinant protein production and purification from Escherichia coli is often accompanied with expensive and complicated procedures, especially for therapeutic proteins. Here it was demonstrated that, by using an intein cleavable polyhydroxyalkanoate synthase fusion, recombinant protein...
Autores principales: | Du, Jinping, Rehm, Bernd H. A. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5667439/ https://www.ncbi.nlm.nih.gov/pubmed/29096637 http://dx.doi.org/10.1186/s12934-017-0799-1 |
Ejemplares similares
-
Polyhydroyxalkanoate Synthase Fusions as a Strategy for Oriented Enzyme Immobilisation
por: Hooks, David O., et al.
Publicado: (2014) -
Inteins and affinity resin substitutes for protein purification and scale up
por: Banki, Mahmoud Reza, et al.
Publicado: (2005) -
Streamlined protein expression and purification using cleavable self-aggregating tags
por: Xing, Lei, et al.
Publicado: (2011) -
Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation
por: Fong, Baley A, et al.
Publicado: (2010) -
In vivo polyester immobilized sortase for tagless protein purification
por: Hay, Iain D., et al.
Publicado: (2015)