Linear and exponential TAIL-PCR: a method for efficient and quick amplification of flanking sequences adjacent to Tn5 transposon insertion sites

Current genome walking methods are very time consuming, and many produce non-specific amplification products. To amplify the flanking sequences that are adjacent to Tn5 transposon insertion sites in Serratia marcescens FZSF02, we developed a genome walking method based on TAIL-PCR. This PCR method a...

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Detalles Bibliográficos
Autores principales: Jia, Xianbo, Lin, Xinjian, Chen, Jichen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668224/
https://www.ncbi.nlm.nih.gov/pubmed/29098449
http://dx.doi.org/10.1186/s13568-017-0495-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668224/
https://www.ncbi.nlm.nih.gov/pubmed/29098449
http://dx.doi.org/10.1186/s13568-017-0495-x

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