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Chromogenic assessment of the three molybdo-selenoprotein formate dehydrogenases in Escherichia coli

Escherichia coli synthesizes three selenocysteine-dependent formate dehydrogenases (Fdh) that also have a molybdenum cofactor. Fdh-H couples formate oxidation with proton reduction in the formate hydrogenlyase (FHL) complex. The activity of Fdh-H in solution can be measured with artificial redox dye...

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Detalles Bibliográficos
Autores principales: Hartwig, Stefanie, Pinske, Constanze, Sawers, R. Gary
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668559/
https://www.ncbi.nlm.nih.gov/pubmed/29124134
http://dx.doi.org/10.1016/j.bbrep.2015.03.006