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High efficiency reduction capability for the formation of Fab׳ antibody fragments from F(ab)(2) units

Antibodies have widespread applications in areas ranging from therapeutics to chromatography and protein microarrays. Certain applications require only the fragment antigen-binding (Fab) units of the protein. This study compares the cleavage efficacy of dithiothreitol (DTT), mercaptoethylamine (MEA)...

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Detalles Bibliográficos
Autores principales: Crivianu-Gaita, Victor, Romaschin, Alexander, Thompson, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668623/
https://www.ncbi.nlm.nih.gov/pubmed/29124142
http://dx.doi.org/10.1016/j.bbrep.2015.04.004