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A method of gentle hydration to prepare oil-free giant unilamellar vesicles that can confine enzymatic reactions
We report a new and improved method to prepare, by gentle hydration of lipid films, oil-free giant unilamellar vesicles (GUVs), in which enzymatic reactions can be encapsulated. The traditional method of gentle hydration requires very low concentrations of metal ions, whereas enzymatic reactions gen...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668676/ https://www.ncbi.nlm.nih.gov/pubmed/29124169 http://dx.doi.org/10.1016/j.bbrep.2015.07.005 |