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A method of gentle hydration to prepare oil-free giant unilamellar vesicles that can confine enzymatic reactions

We report a new and improved method to prepare, by gentle hydration of lipid films, oil-free giant unilamellar vesicles (GUVs), in which enzymatic reactions can be encapsulated. The traditional method of gentle hydration requires very low concentrations of metal ions, whereas enzymatic reactions gen...

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Detalles Bibliográficos
Autores principales: Shohda, K., Takahashi, K., Suyama, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668676/
https://www.ncbi.nlm.nih.gov/pubmed/29124169
http://dx.doi.org/10.1016/j.bbrep.2015.07.005