Optimizing hyaluronidase dose and plasmid DNA delivery greatly improves gene electrotransfer efficiency in rat skeletal muscle

Transfection of rat skeletal muscle in vivo is a widely used research model. However, gene electrotransfer protocols have been developed for mice and yield variable results in rats. We investigated whether changes in hyaluronidase pre-treatment and plasmid DNA delivery can improve transfection effic...

Descripción completa

Detalles Bibliográficos
Autores principales: Akerstrom, Thorbjorn, Vedel, Kenneth, Needham, Josefine, Hojman, Pernille, Kontou, Eftychia, Hellsten, Ylva, Wojtaszewski, Jørgen F.P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669402/
https://www.ncbi.nlm.nih.gov/pubmed/29124223
http://dx.doi.org/10.1016/j.bbrep.2015.10.007

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669402/
https://www.ncbi.nlm.nih.gov/pubmed/29124223
http://dx.doi.org/10.1016/j.bbrep.2015.10.007

Ejemplares similares