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Optimizing hyaluronidase dose and plasmid DNA delivery greatly improves gene electrotransfer efficiency in rat skeletal muscle

Transfection of rat skeletal muscle in vivo is a widely used research model. However, gene electrotransfer protocols have been developed for mice and yield variable results in rats. We investigated whether changes in hyaluronidase pre-treatment and plasmid DNA delivery can improve transfection effic...

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Detalles Bibliográficos
Autores principales:	Akerstrom, Thorbjorn, Vedel, Kenneth, Needham, Josefine, Hojman, Pernille, Kontou, Eftychia, Hellsten, Ylva, Wojtaszewski, Jørgen F.P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669402/ https://www.ncbi.nlm.nih.gov/pubmed/29124223 http://dx.doi.org/10.1016/j.bbrep.2015.10.007

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