Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays

Among the triterpenoids, oleanolic acid (OA) and its isomer, ursolic acid (UA) are promising therapeutic candidates, with potential benefits in the management of melanoma. In this study, we aimed to examine the in vitro and in vivo anti-invasive and anti-metastatic activity of OA and UA to determine...

Descripción completa

Detalles Bibliográficos
Autores principales: Caunii, Angela, Oprean, Camelia, Cristea, Mirabela, Ivan, Alexandra, Danciu, Corina, Tatu, Calin, Paunescu, Virgil, Marti, Daniela, Tzanakakis, George, Spandidos, Demetrios A., Tsatsakis, Aristides, Susan, Razvan, Soica, Codruta, Avram, Stefana, Dehelean, Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673023/
https://www.ncbi.nlm.nih.gov/pubmed/29039461
http://dx.doi.org/10.3892/ijo.2017.4160
_version_ 1783276536073289728
author Caunii, Angela
Oprean, Camelia
Cristea, Mirabela
Ivan, Alexandra
Danciu, Corina
Tatu, Calin
Paunescu, Virgil
Marti, Daniela
Tzanakakis, George
Spandidos, Demetrios A.
Tsatsakis, Aristides
Susan, Razvan
Soica, Codruta
Avram, Stefana
Dehelean, Cristina
author_facet Caunii, Angela
Oprean, Camelia
Cristea, Mirabela
Ivan, Alexandra
Danciu, Corina
Tatu, Calin
Paunescu, Virgil
Marti, Daniela
Tzanakakis, George
Spandidos, Demetrios A.
Tsatsakis, Aristides
Susan, Razvan
Soica, Codruta
Avram, Stefana
Dehelean, Cristina
author_sort Caunii, Angela
collection PubMed
description Among the triterpenoids, oleanolic acid (OA) and its isomer, ursolic acid (UA) are promising therapeutic candidates, with potential benefits in the management of melanoma. In this study, we aimed to examine the in vitro and in vivo anti-invasive and anti-metastatic activity of OA and UA to determine their possible usefulness as chemopreventive or chemotherapeutic agents in melanoma. For the in vitro experiments, the anti-proliferative activity of the triterpenic compounds on SK-MEL-2 melanoma cells was examined. The anti-invasive potential was assessed by testing the effects of the active compound on vascular cell adhesion molecule (VCAM) and intercellular adhesion molecule (ICAM) adhesion to melanoma cells. Normal and tumor angiogenesis were evaluated in vivo by chicken embryo chorioallantoic membrane (CAM) assay. The two test triterpenoid acids, UA and OA, exerted differential effects in vitro and in vivo on the SK-MEL-2 melanoma cells. UA exerted a significant and dose-dependent anti-proliferative effect in vitro, compared to OA. The cytotoxic effects in vitro on the melanoma cells were determined by the examining alterations in the cell cycle phases induced by UA that lead to cell arrest in the S phase. Moreover, UA was found to affect SK-MEL-2 melanoma cell invasiveness by limiting the cell adhesion capacity to ICAM molecules, but not influencing their adhesion to VCAM molecules. On the whole, in this study, by assessing the effects of the two triterpenoids in vivo, our results revealed that OA had a greater potential to impair the invasive capacity and tumor angiogenesis compared with UA.
format Online
Article
Text
id pubmed-5673023
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-56730232017-11-16 Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays Caunii, Angela Oprean, Camelia Cristea, Mirabela Ivan, Alexandra Danciu, Corina Tatu, Calin Paunescu, Virgil Marti, Daniela Tzanakakis, George Spandidos, Demetrios A. Tsatsakis, Aristides Susan, Razvan Soica, Codruta Avram, Stefana Dehelean, Cristina Int J Oncol Articles Among the triterpenoids, oleanolic acid (OA) and its isomer, ursolic acid (UA) are promising therapeutic candidates, with potential benefits in the management of melanoma. In this study, we aimed to examine the in vitro and in vivo anti-invasive and anti-metastatic activity of OA and UA to determine their possible usefulness as chemopreventive or chemotherapeutic agents in melanoma. For the in vitro experiments, the anti-proliferative activity of the triterpenic compounds on SK-MEL-2 melanoma cells was examined. The anti-invasive potential was assessed by testing the effects of the active compound on vascular cell adhesion molecule (VCAM) and intercellular adhesion molecule (ICAM) adhesion to melanoma cells. Normal and tumor angiogenesis were evaluated in vivo by chicken embryo chorioallantoic membrane (CAM) assay. The two test triterpenoid acids, UA and OA, exerted differential effects in vitro and in vivo on the SK-MEL-2 melanoma cells. UA exerted a significant and dose-dependent anti-proliferative effect in vitro, compared to OA. The cytotoxic effects in vitro on the melanoma cells were determined by the examining alterations in the cell cycle phases induced by UA that lead to cell arrest in the S phase. Moreover, UA was found to affect SK-MEL-2 melanoma cell invasiveness by limiting the cell adhesion capacity to ICAM molecules, but not influencing their adhesion to VCAM molecules. On the whole, in this study, by assessing the effects of the two triterpenoids in vivo, our results revealed that OA had a greater potential to impair the invasive capacity and tumor angiogenesis compared with UA. D.A. Spandidos 2017-10-16 /pmc/articles/PMC5673023/ /pubmed/29039461 http://dx.doi.org/10.3892/ijo.2017.4160 Text en Copyright: © Caunii et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Caunii, Angela
Oprean, Camelia
Cristea, Mirabela
Ivan, Alexandra
Danciu, Corina
Tatu, Calin
Paunescu, Virgil
Marti, Daniela
Tzanakakis, George
Spandidos, Demetrios A.
Tsatsakis, Aristides
Susan, Razvan
Soica, Codruta
Avram, Stefana
Dehelean, Cristina
Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays
title Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays
title_full Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays
title_fullStr Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays
title_full_unstemmed Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays
title_short Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays
title_sort effects of ursolic and oleanolic on sk-mel-2 melanoma cells: in vitro and in vivo assays
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673023/
https://www.ncbi.nlm.nih.gov/pubmed/29039461
http://dx.doi.org/10.3892/ijo.2017.4160
work_keys_str_mv AT cauniiangela effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT opreancamelia effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT cristeamirabela effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT ivanalexandra effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT danciucorina effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT tatucalin effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT paunescuvirgil effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT martidaniela effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT tzanakakisgeorge effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT spandidosdemetriosa effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT tsatsakisaristides effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT susanrazvan effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT soicacodruta effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT avramstefana effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays
AT deheleancristina effectsofursolicandoleanoliconskmel2melanomacellsinvitroandinvivoassays