Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays
Among the triterpenoids, oleanolic acid (OA) and its isomer, ursolic acid (UA) are promising therapeutic candidates, with potential benefits in the management of melanoma. In this study, we aimed to examine the in vitro and in vivo anti-invasive and anti-metastatic activity of OA and UA to determine...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673023/ https://www.ncbi.nlm.nih.gov/pubmed/29039461 http://dx.doi.org/10.3892/ijo.2017.4160 |
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author | Caunii, Angela Oprean, Camelia Cristea, Mirabela Ivan, Alexandra Danciu, Corina Tatu, Calin Paunescu, Virgil Marti, Daniela Tzanakakis, George Spandidos, Demetrios A. Tsatsakis, Aristides Susan, Razvan Soica, Codruta Avram, Stefana Dehelean, Cristina |
author_facet | Caunii, Angela Oprean, Camelia Cristea, Mirabela Ivan, Alexandra Danciu, Corina Tatu, Calin Paunescu, Virgil Marti, Daniela Tzanakakis, George Spandidos, Demetrios A. Tsatsakis, Aristides Susan, Razvan Soica, Codruta Avram, Stefana Dehelean, Cristina |
author_sort | Caunii, Angela |
collection | PubMed |
description | Among the triterpenoids, oleanolic acid (OA) and its isomer, ursolic acid (UA) are promising therapeutic candidates, with potential benefits in the management of melanoma. In this study, we aimed to examine the in vitro and in vivo anti-invasive and anti-metastatic activity of OA and UA to determine their possible usefulness as chemopreventive or chemotherapeutic agents in melanoma. For the in vitro experiments, the anti-proliferative activity of the triterpenic compounds on SK-MEL-2 melanoma cells was examined. The anti-invasive potential was assessed by testing the effects of the active compound on vascular cell adhesion molecule (VCAM) and intercellular adhesion molecule (ICAM) adhesion to melanoma cells. Normal and tumor angiogenesis were evaluated in vivo by chicken embryo chorioallantoic membrane (CAM) assay. The two test triterpenoid acids, UA and OA, exerted differential effects in vitro and in vivo on the SK-MEL-2 melanoma cells. UA exerted a significant and dose-dependent anti-proliferative effect in vitro, compared to OA. The cytotoxic effects in vitro on the melanoma cells were determined by the examining alterations in the cell cycle phases induced by UA that lead to cell arrest in the S phase. Moreover, UA was found to affect SK-MEL-2 melanoma cell invasiveness by limiting the cell adhesion capacity to ICAM molecules, but not influencing their adhesion to VCAM molecules. On the whole, in this study, by assessing the effects of the two triterpenoids in vivo, our results revealed that OA had a greater potential to impair the invasive capacity and tumor angiogenesis compared with UA. |
format | Online Article Text |
id | pubmed-5673023 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-56730232017-11-16 Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays Caunii, Angela Oprean, Camelia Cristea, Mirabela Ivan, Alexandra Danciu, Corina Tatu, Calin Paunescu, Virgil Marti, Daniela Tzanakakis, George Spandidos, Demetrios A. Tsatsakis, Aristides Susan, Razvan Soica, Codruta Avram, Stefana Dehelean, Cristina Int J Oncol Articles Among the triterpenoids, oleanolic acid (OA) and its isomer, ursolic acid (UA) are promising therapeutic candidates, with potential benefits in the management of melanoma. In this study, we aimed to examine the in vitro and in vivo anti-invasive and anti-metastatic activity of OA and UA to determine their possible usefulness as chemopreventive or chemotherapeutic agents in melanoma. For the in vitro experiments, the anti-proliferative activity of the triterpenic compounds on SK-MEL-2 melanoma cells was examined. The anti-invasive potential was assessed by testing the effects of the active compound on vascular cell adhesion molecule (VCAM) and intercellular adhesion molecule (ICAM) adhesion to melanoma cells. Normal and tumor angiogenesis were evaluated in vivo by chicken embryo chorioallantoic membrane (CAM) assay. The two test triterpenoid acids, UA and OA, exerted differential effects in vitro and in vivo on the SK-MEL-2 melanoma cells. UA exerted a significant and dose-dependent anti-proliferative effect in vitro, compared to OA. The cytotoxic effects in vitro on the melanoma cells were determined by the examining alterations in the cell cycle phases induced by UA that lead to cell arrest in the S phase. Moreover, UA was found to affect SK-MEL-2 melanoma cell invasiveness by limiting the cell adhesion capacity to ICAM molecules, but not influencing their adhesion to VCAM molecules. On the whole, in this study, by assessing the effects of the two triterpenoids in vivo, our results revealed that OA had a greater potential to impair the invasive capacity and tumor angiogenesis compared with UA. D.A. Spandidos 2017-10-16 /pmc/articles/PMC5673023/ /pubmed/29039461 http://dx.doi.org/10.3892/ijo.2017.4160 Text en Copyright: © Caunii et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Caunii, Angela Oprean, Camelia Cristea, Mirabela Ivan, Alexandra Danciu, Corina Tatu, Calin Paunescu, Virgil Marti, Daniela Tzanakakis, George Spandidos, Demetrios A. Tsatsakis, Aristides Susan, Razvan Soica, Codruta Avram, Stefana Dehelean, Cristina Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays |
title | Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays |
title_full | Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays |
title_fullStr | Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays |
title_full_unstemmed | Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays |
title_short | Effects of ursolic and oleanolic on SK-MEL-2 melanoma cells: In vitro and in vivo assays |
title_sort | effects of ursolic and oleanolic on sk-mel-2 melanoma cells: in vitro and in vivo assays |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673023/ https://www.ncbi.nlm.nih.gov/pubmed/29039461 http://dx.doi.org/10.3892/ijo.2017.4160 |
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