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Serial-omics of P53−/−, Brca1−/− Mouse Breast Tumor and Normal Mammary Gland

This study demonstrates a liquid-liquid extraction for the sequential tandem mass spectrometry (LC-MS/MS) analysis of non-polar lipids, polar metabolites, proteins and phosphorylation sites from a single piece of tissue. Extraction of 10 mg BRCA−/−, p53−/− breast tumor tissue or normal mammary gland...

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Detalles Bibliográficos
Autores principales: Breitkopf, Susanne B., Taveira, Mateus De Oliveira, Yuan, Min, Wulf, Gerburg M., Asara, John M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674068/
https://www.ncbi.nlm.nih.gov/pubmed/29109428
http://dx.doi.org/10.1038/s41598-017-15132-y