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A Novel Affinity Tag, ABTAG, and Its Application to the Affinity Screening of Single-Domain Antibodies Selected by Phage Display
ABTAG is a camelid single-domain antibody (sdAb) that binds to bovine serum albumin (BSA) with low picomolar affinity. In surface plasmon resonance (SPR) analyses using BSA surfaces, bound ABTAG can be completely dissociated from the BSA surfaces at low pH, over multiple cycles, without any reductio...
Autores principales: | Hussack, Greg, Baral, Toya Nath, Baardsnes, Jason, van Faassen, Henk, Raphael, Shalini, Henry, Kevin A., Zhang, Jianbing, MacKenzie, C. Roger |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674936/ https://www.ncbi.nlm.nih.gov/pubmed/29163485 http://dx.doi.org/10.3389/fimmu.2017.01406 |
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