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Single primer isothermal amplification (SPIA) combined with next generation sequencing provides complete bovine coronavirus genome coverage and higher sequence depth compared to sequence-independent single primer amplification (SISPA)

Coronaviruses are of major importance for both animal and human health. With the emergence of novel coronaviruses such as SARS and MERS, the need for fast genome characterisation is ever so important. Further, in order to understand the influence of quasispecies of these viruses in relation to biolo...

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Detalles Bibliográficos
Autores principales: Myrmel, Mette, Oma, Veslemøy, Khatri, Mamata, Hansen, Hanne H., Stokstad, Maria, Berg, Mikael, Blomström, Anne-Lie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5675387/
https://www.ncbi.nlm.nih.gov/pubmed/29112950
http://dx.doi.org/10.1371/journal.pone.0187780