Polymorphisms in CCR5Δ32 and Risk of HIV-1 Infection in the Southeast of Caspian Sea, Iran

Prevalence of CCR5Δ32 among blood samples of more than 400 healthy and HIV-1-infected people was investigated in Iran. Polymerase chain reaction (PCR) following DNA extraction was used. Desired frequency was analyzed by Hardy–Weinberg equilibrium (HWE) analysis and SPSS 16.0 software to harvest the...

Descripción completa

Detalles Bibliográficos
Autores principales: Heydarifard, Zahra, Tabarraei, Alijan, Moradi, Abdolvahab
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676439/
https://www.ncbi.nlm.nih.gov/pubmed/29209099
http://dx.doi.org/10.1155/2017/4190107
_version_ 1783277068438470656
author Heydarifard, Zahra
Tabarraei, Alijan
Moradi, Abdolvahab
author_facet Heydarifard, Zahra
Tabarraei, Alijan
Moradi, Abdolvahab
author_sort Heydarifard, Zahra
collection PubMed
description Prevalence of CCR5Δ32 among blood samples of more than 400 healthy and HIV-1-infected people was investigated in Iran. Polymerase chain reaction (PCR) following DNA extraction was used. Desired frequency was analyzed by Hardy–Weinberg equilibrium (HWE) analysis and SPSS 16.0 software to harvest the results. The prevalence of CCRΔ32 heterozygote genotype was 3% in healthy people and 0.7% in HIV-1–infected individuals. There was no homozygote CCR5Δ32 in both groups, and the allele Δ32 was only observed in 1.5% and 0.36% of healthy and HIV-1–infected participants, respectively. Therefore according to this study, the frequency of the allele CCR5Δ32 indicates no significant difference between either groups (p = 0.18) and it sounds that the mentioned mutation in heterozygote people would not affect their susceptibility against HIV infection. Genotyping trial in Iranians with HIV infection is supposed to be helpful as a matter of prognostic purposes.
format Online
Article
Text
id pubmed-5676439
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-56764392017-12-05 Polymorphisms in CCR5Δ32 and Risk of HIV-1 Infection in the Southeast of Caspian Sea, Iran Heydarifard, Zahra Tabarraei, Alijan Moradi, Abdolvahab Dis Markers Research Article Prevalence of CCR5Δ32 among blood samples of more than 400 healthy and HIV-1-infected people was investigated in Iran. Polymerase chain reaction (PCR) following DNA extraction was used. Desired frequency was analyzed by Hardy–Weinberg equilibrium (HWE) analysis and SPSS 16.0 software to harvest the results. The prevalence of CCRΔ32 heterozygote genotype was 3% in healthy people and 0.7% in HIV-1–infected individuals. There was no homozygote CCR5Δ32 in both groups, and the allele Δ32 was only observed in 1.5% and 0.36% of healthy and HIV-1–infected participants, respectively. Therefore according to this study, the frequency of the allele CCR5Δ32 indicates no significant difference between either groups (p = 0.18) and it sounds that the mentioned mutation in heterozygote people would not affect their susceptibility against HIV infection. Genotyping trial in Iranians with HIV infection is supposed to be helpful as a matter of prognostic purposes. Hindawi 2017 2017-10-25 /pmc/articles/PMC5676439/ /pubmed/29209099 http://dx.doi.org/10.1155/2017/4190107 Text en Copyright © 2017 Zahra Heydarifard et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Heydarifard, Zahra
Tabarraei, Alijan
Moradi, Abdolvahab
Polymorphisms in CCR5Δ32 and Risk of HIV-1 Infection in the Southeast of Caspian Sea, Iran
title Polymorphisms in CCR5Δ32 and Risk of HIV-1 Infection in the Southeast of Caspian Sea, Iran
title_full Polymorphisms in CCR5Δ32 and Risk of HIV-1 Infection in the Southeast of Caspian Sea, Iran
title_fullStr Polymorphisms in CCR5Δ32 and Risk of HIV-1 Infection in the Southeast of Caspian Sea, Iran
title_full_unstemmed Polymorphisms in CCR5Δ32 and Risk of HIV-1 Infection in the Southeast of Caspian Sea, Iran
title_short Polymorphisms in CCR5Δ32 and Risk of HIV-1 Infection in the Southeast of Caspian Sea, Iran
title_sort polymorphisms in ccr5δ32 and risk of hiv-1 infection in the southeast of caspian sea, iran
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676439/
https://www.ncbi.nlm.nih.gov/pubmed/29209099
http://dx.doi.org/10.1155/2017/4190107
work_keys_str_mv AT heydarifardzahra polymorphismsinccr5d32andriskofhiv1infectioninthesoutheastofcaspianseairan
AT tabarraeialijan polymorphismsinccr5d32andriskofhiv1infectioninthesoutheastofcaspianseairan
AT moradiabdolvahab polymorphismsinccr5d32andriskofhiv1infectioninthesoutheastofcaspianseairan