A method for measuring the distribution of the shortest telomeres in cells and tissues

Improved methods to measure the shortest (not just average) telomere lengths (TLs) are needed. We developed Telomere Shortest Length Assay (TeSLA), a technique that detects telomeres from all chromosome ends from <1 kb to 18 kb using small amounts of input DNA. TeSLA improves the specificity and...

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Autores principales: Lai, Tsung-Po, Zhang, Ning, Noh, Jungsik, Mender, Ilgen, Tedone, Enzo, Huang, Ejun, Wright, Woodring E., Danuser, Gaudenz, Shay, Jerry W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676791/
https://www.ncbi.nlm.nih.gov/pubmed/29116081
http://dx.doi.org/10.1038/s41467-017-01291-z
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author Lai, Tsung-Po
Zhang, Ning
Noh, Jungsik
Mender, Ilgen
Tedone, Enzo
Huang, Ejun
Wright, Woodring E.
Danuser, Gaudenz
Shay, Jerry W.
author_facet Lai, Tsung-Po
Zhang, Ning
Noh, Jungsik
Mender, Ilgen
Tedone, Enzo
Huang, Ejun
Wright, Woodring E.
Danuser, Gaudenz
Shay, Jerry W.
author_sort Lai, Tsung-Po
collection PubMed
description Improved methods to measure the shortest (not just average) telomere lengths (TLs) are needed. We developed Telomere Shortest Length Assay (TeSLA), a technique that detects telomeres from all chromosome ends from <1 kb to 18 kb using small amounts of input DNA. TeSLA improves the specificity and efficiency of TL measurements that is facilitated by user friendly image-processing software to automatically detect and annotate band sizes, calculate average TL, as well as the percent of the shortest telomeres. Compared with other TL measurement methods, TeSLA provides more information about the shortest telomeres. The length of telomeres was measured longitudinally in peripheral blood mononuclear cells during human aging, in tissues during colon cancer progression, in telomere-related diseases such as idiopathic pulmonary fibrosis, as well as in mice and other organisms. The results indicate that TeSLA is a robust method that provides a better understanding of the shortest length of telomeres.
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spelling pubmed-56767912017-11-13 A method for measuring the distribution of the shortest telomeres in cells and tissues Lai, Tsung-Po Zhang, Ning Noh, Jungsik Mender, Ilgen Tedone, Enzo Huang, Ejun Wright, Woodring E. Danuser, Gaudenz Shay, Jerry W. Nat Commun Article Improved methods to measure the shortest (not just average) telomere lengths (TLs) are needed. We developed Telomere Shortest Length Assay (TeSLA), a technique that detects telomeres from all chromosome ends from <1 kb to 18 kb using small amounts of input DNA. TeSLA improves the specificity and efficiency of TL measurements that is facilitated by user friendly image-processing software to automatically detect and annotate band sizes, calculate average TL, as well as the percent of the shortest telomeres. Compared with other TL measurement methods, TeSLA provides more information about the shortest telomeres. The length of telomeres was measured longitudinally in peripheral blood mononuclear cells during human aging, in tissues during colon cancer progression, in telomere-related diseases such as idiopathic pulmonary fibrosis, as well as in mice and other organisms. The results indicate that TeSLA is a robust method that provides a better understanding of the shortest length of telomeres. Nature Publishing Group UK 2017-11-07 /pmc/articles/PMC5676791/ /pubmed/29116081 http://dx.doi.org/10.1038/s41467-017-01291-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lai, Tsung-Po
Zhang, Ning
Noh, Jungsik
Mender, Ilgen
Tedone, Enzo
Huang, Ejun
Wright, Woodring E.
Danuser, Gaudenz
Shay, Jerry W.
A method for measuring the distribution of the shortest telomeres in cells and tissues
title A method for measuring the distribution of the shortest telomeres in cells and tissues
title_full A method for measuring the distribution of the shortest telomeres in cells and tissues
title_fullStr A method for measuring the distribution of the shortest telomeres in cells and tissues
title_full_unstemmed A method for measuring the distribution of the shortest telomeres in cells and tissues
title_short A method for measuring the distribution of the shortest telomeres in cells and tissues
title_sort method for measuring the distribution of the shortest telomeres in cells and tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676791/
https://www.ncbi.nlm.nih.gov/pubmed/29116081
http://dx.doi.org/10.1038/s41467-017-01291-z
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