Highly Efficient, Rapid and Co-CRISPR-Independent Genome Editing in Caenorhabditis elegans

We describe a rapid and highly efficient method to generate point mutations in Caenorhabditis elegans using direct injection of CRISPR-Cas9 ribonucleoproteins. This versatile method does not require sensitized genetic backgrounds or co-CRISPR selection-based methods, and represents a single strategy...

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Detalles Bibliográficos
Autores principales: Prior, Harriet, Jawad, Ali K., MacConnachie, Lauren, Beg, Asim A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2017
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677160/
https://www.ncbi.nlm.nih.gov/pubmed/28893845
http://dx.doi.org/10.1534/g3.117.300216

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677160/
https://www.ncbi.nlm.nih.gov/pubmed/28893845
http://dx.doi.org/10.1534/g3.117.300216

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