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Asian Zika virus strains target CD14(+) blood monocytes and induce M2-skewed immunosuppression during pregnancy
Blood CD14(+) monocytes are the frontline immunomodulators categorized into classical, intermediate or non-classical subsets, subsequently differentiating into M1 pro- or M2 anti-inflammatory macrophages upon stimulation. While Zika virus (ZIKV) rapidly establishes viremia, the target cells and immu...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5678934/ https://www.ncbi.nlm.nih.gov/pubmed/28827581 http://dx.doi.org/10.1038/s41564-017-0016-3 |
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author | Foo, Suan-Sin Chen, Weiqiang Chan, Yen Bowman, James W. Chang, Lin-Chun Choi, Younho Yoo, Ji Seung Ge, Jianning Cheng, Genhong Bonnin, Alexandre Nielsen-Saines, Karin Brasil, Patrícia Jung, Jae U. |
author_facet | Foo, Suan-Sin Chen, Weiqiang Chan, Yen Bowman, James W. Chang, Lin-Chun Choi, Younho Yoo, Ji Seung Ge, Jianning Cheng, Genhong Bonnin, Alexandre Nielsen-Saines, Karin Brasil, Patrícia Jung, Jae U. |
author_sort | Foo, Suan-Sin |
collection | PubMed |
description | Blood CD14(+) monocytes are the frontline immunomodulators categorized into classical, intermediate or non-classical subsets, subsequently differentiating into M1 pro- or M2 anti-inflammatory macrophages upon stimulation. While Zika virus (ZIKV) rapidly establishes viremia, the target cells and immune responses, particularly during pregnancy, remain elusive. Furthermore, it is unknown whether African- and Asian-lineage ZIKV have different phenotypic impacts on host immune responses. Using human blood infection, we identified CD14(+) monocytes as the primary target for African- or Asian-lineage ZIKV infection. When immunoprofiles of human blood infected with ZIKV were compared, a classical/intermediate monocyte-mediated M1-skewed inflammation by African-lineage ZIKV infection was observed, in contrast to a non-classical monocyte-mediated M2-skewed immunosuppression by Asian-lineage ZIKV infection. Importantly, infection of pregnant women’s blood revealed enhanced susceptibility to ZIKV infection. Specifically, Asian-lineage ZIKV infection of pregnant women’s blood led to an exacerbated M2-skewed immunosuppression of non-classical monocytes in conjunction with global suppression of type I interferon-signaling pathway and an aberrant expression of host genes associated with pregnancy complications. 30 ZIKV(+) sera from symptomatic pregnant patients also showed elevated levels of M2-skewed immunosuppressive cytokines and pregnancy complication-associated fibronectin-1. This study demonstrates the differential immunomodulatory responses of blood monocytes, particularly during pregnancy, upon infection with different lineages of ZIKV. |
format | Online Article Text |
id | pubmed-5678934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
record_format | MEDLINE/PubMed |
spelling | pubmed-56789342018-02-21 Asian Zika virus strains target CD14(+) blood monocytes and induce M2-skewed immunosuppression during pregnancy Foo, Suan-Sin Chen, Weiqiang Chan, Yen Bowman, James W. Chang, Lin-Chun Choi, Younho Yoo, Ji Seung Ge, Jianning Cheng, Genhong Bonnin, Alexandre Nielsen-Saines, Karin Brasil, Patrícia Jung, Jae U. Nat Microbiol Article Blood CD14(+) monocytes are the frontline immunomodulators categorized into classical, intermediate or non-classical subsets, subsequently differentiating into M1 pro- or M2 anti-inflammatory macrophages upon stimulation. While Zika virus (ZIKV) rapidly establishes viremia, the target cells and immune responses, particularly during pregnancy, remain elusive. Furthermore, it is unknown whether African- and Asian-lineage ZIKV have different phenotypic impacts on host immune responses. Using human blood infection, we identified CD14(+) monocytes as the primary target for African- or Asian-lineage ZIKV infection. When immunoprofiles of human blood infected with ZIKV were compared, a classical/intermediate monocyte-mediated M1-skewed inflammation by African-lineage ZIKV infection was observed, in contrast to a non-classical monocyte-mediated M2-skewed immunosuppression by Asian-lineage ZIKV infection. Importantly, infection of pregnant women’s blood revealed enhanced susceptibility to ZIKV infection. Specifically, Asian-lineage ZIKV infection of pregnant women’s blood led to an exacerbated M2-skewed immunosuppression of non-classical monocytes in conjunction with global suppression of type I interferon-signaling pathway and an aberrant expression of host genes associated with pregnancy complications. 30 ZIKV(+) sera from symptomatic pregnant patients also showed elevated levels of M2-skewed immunosuppressive cytokines and pregnancy complication-associated fibronectin-1. This study demonstrates the differential immunomodulatory responses of blood monocytes, particularly during pregnancy, upon infection with different lineages of ZIKV. 2017-08-21 2017-11 /pmc/articles/PMC5678934/ /pubmed/28827581 http://dx.doi.org/10.1038/s41564-017-0016-3 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Foo, Suan-Sin Chen, Weiqiang Chan, Yen Bowman, James W. Chang, Lin-Chun Choi, Younho Yoo, Ji Seung Ge, Jianning Cheng, Genhong Bonnin, Alexandre Nielsen-Saines, Karin Brasil, Patrícia Jung, Jae U. Asian Zika virus strains target CD14(+) blood monocytes and induce M2-skewed immunosuppression during pregnancy |
title | Asian Zika virus strains target CD14(+) blood monocytes and induce M2-skewed immunosuppression during pregnancy |
title_full | Asian Zika virus strains target CD14(+) blood monocytes and induce M2-skewed immunosuppression during pregnancy |
title_fullStr | Asian Zika virus strains target CD14(+) blood monocytes and induce M2-skewed immunosuppression during pregnancy |
title_full_unstemmed | Asian Zika virus strains target CD14(+) blood monocytes and induce M2-skewed immunosuppression during pregnancy |
title_short | Asian Zika virus strains target CD14(+) blood monocytes and induce M2-skewed immunosuppression during pregnancy |
title_sort | asian zika virus strains target cd14(+) blood monocytes and induce m2-skewed immunosuppression during pregnancy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5678934/ https://www.ncbi.nlm.nih.gov/pubmed/28827581 http://dx.doi.org/10.1038/s41564-017-0016-3 |
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