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Achieving better than 3 Å resolution by single particle cryo-EM at 200 keV

Nearly 98% of single particle cryo-EM structures resolved to better than 4 Å resolution have been determined using 300 keV transmission electron microscopes. We demonstrate that it is possible to obtain reconstructions of macromolecular complexes at a range of sizes to better than 3 Å resolution usi...

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Detalles Bibliográficos
Autores principales: Herzik, Mark A., Wu, Mengyu, Lander, Gabriel C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679434/
https://www.ncbi.nlm.nih.gov/pubmed/28991891
http://dx.doi.org/10.1038/nmeth.4461