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Achieving better than 3 Å resolution by single particle cryo-EM at 200 keV
Nearly 98% of single particle cryo-EM structures resolved to better than 4 Å resolution have been determined using 300 keV transmission electron microscopes. We demonstrate that it is possible to obtain reconstructions of macromolecular complexes at a range of sizes to better than 3 Å resolution usi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679434/ https://www.ncbi.nlm.nih.gov/pubmed/28991891 http://dx.doi.org/10.1038/nmeth.4461 |