Achieving better than 3 Å resolution by single particle cryo-EM at 200 keV

Nearly 98% of single particle cryo-EM structures resolved to better than 4 Å resolution have been determined using 300 keV transmission electron microscopes. We demonstrate that it is possible to obtain reconstructions of macromolecular complexes at a range of sizes to better than 3 Å resolution usi...

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Autores principales: Herzik, Mark A., Wu, Mengyu, Lander, Gabriel C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679434/
https://www.ncbi.nlm.nih.gov/pubmed/28991891
http://dx.doi.org/10.1038/nmeth.4461
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author Herzik, Mark A.
Wu, Mengyu
Lander, Gabriel C.
author_facet Herzik, Mark A.
Wu, Mengyu
Lander, Gabriel C.
author_sort Herzik, Mark A.
collection PubMed
description Nearly 98% of single particle cryo-EM structures resolved to better than 4 Å resolution have been determined using 300 keV transmission electron microscopes. We demonstrate that it is possible to obtain reconstructions of macromolecular complexes at a range of sizes to better than 3 Å resolution using a 200 keV transmission electron microscope. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules.
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spelling pubmed-56794342018-04-09 Achieving better than 3 Å resolution by single particle cryo-EM at 200 keV Herzik, Mark A. Wu, Mengyu Lander, Gabriel C. Nat Methods Article Nearly 98% of single particle cryo-EM structures resolved to better than 4 Å resolution have been determined using 300 keV transmission electron microscopes. We demonstrate that it is possible to obtain reconstructions of macromolecular complexes at a range of sizes to better than 3 Å resolution using a 200 keV transmission electron microscope. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules. 2017-10-09 2017-11 /pmc/articles/PMC5679434/ /pubmed/28991891 http://dx.doi.org/10.1038/nmeth.4461 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Herzik, Mark A.
Wu, Mengyu
Lander, Gabriel C.
Achieving better than 3 Å resolution by single particle cryo-EM at 200 keV
title Achieving better than 3 Å resolution by single particle cryo-EM at 200 keV
title_full Achieving better than 3 Å resolution by single particle cryo-EM at 200 keV
title_fullStr Achieving better than 3 Å resolution by single particle cryo-EM at 200 keV
title_full_unstemmed Achieving better than 3 Å resolution by single particle cryo-EM at 200 keV
title_short Achieving better than 3 Å resolution by single particle cryo-EM at 200 keV
title_sort achieving better than 3 å resolution by single particle cryo-em at 200 kev
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679434/
https://www.ncbi.nlm.nih.gov/pubmed/28991891
http://dx.doi.org/10.1038/nmeth.4461
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