Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis

The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe “semisynthetic” pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Ora...

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Detalles Bibliográficos
Autores principales: Martineau, Magalie, Somasundaram, Agila, Grimm, Jonathan B., Gruber, Todd D., Choquet, Daniel, Taraska, Justin W., Lavis, Luke D., Perrais, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5680258/
https://www.ncbi.nlm.nih.gov/pubmed/29123102
http://dx.doi.org/10.1038/s41467-017-01752-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5680258/
https://www.ncbi.nlm.nih.gov/pubmed/29123102
http://dx.doi.org/10.1038/s41467-017-01752-5

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