Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis

The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe “semisynthetic” pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Ora...

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Autores principales: Martineau, Magalie, Somasundaram, Agila, Grimm, Jonathan B., Gruber, Todd D., Choquet, Daniel, Taraska, Justin W., Lavis, Luke D., Perrais, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5680258/
https://www.ncbi.nlm.nih.gov/pubmed/29123102
http://dx.doi.org/10.1038/s41467-017-01752-5
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author Martineau, Magalie
Somasundaram, Agila
Grimm, Jonathan B.
Gruber, Todd D.
Choquet, Daniel
Taraska, Justin W.
Lavis, Luke D.
Perrais, David
author_facet Martineau, Magalie
Somasundaram, Agila
Grimm, Jonathan B.
Gruber, Todd D.
Choquet, Daniel
Taraska, Justin W.
Lavis, Luke D.
Perrais, David
author_sort Martineau, Magalie
collection PubMed
description The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe “semisynthetic” pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.
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spelling pubmed-56802582017-11-15 Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis Martineau, Magalie Somasundaram, Agila Grimm, Jonathan B. Gruber, Todd D. Choquet, Daniel Taraska, Justin W. Lavis, Luke D. Perrais, David Nat Commun Article The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe “semisynthetic” pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission. Nature Publishing Group UK 2017-11-10 /pmc/articles/PMC5680258/ /pubmed/29123102 http://dx.doi.org/10.1038/s41467-017-01752-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Martineau, Magalie
Somasundaram, Agila
Grimm, Jonathan B.
Gruber, Todd D.
Choquet, Daniel
Taraska, Justin W.
Lavis, Luke D.
Perrais, David
Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
title Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
title_full Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
title_fullStr Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
title_full_unstemmed Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
title_short Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
title_sort semisynthetic fluorescent ph sensors for imaging exocytosis and endocytosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5680258/
https://www.ncbi.nlm.nih.gov/pubmed/29123102
http://dx.doi.org/10.1038/s41467-017-01752-5
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