Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies

Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease. We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EV...

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Detalles Bibliográficos
Autores principales: Benedikter, Birke J., Bouwman, Freek G., Vajen, Tanja, Heinzmann, Alexandra C. A., Grauls, Gert, Mariman, Edwin C., Wouters, Emiel F. M., Savelkoul, Paul H., Lopez-Iglesias, Carmen, Koenen, Rory R., Rohde, Gernot G. U., Stassen, Frank R. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5681555/
https://www.ncbi.nlm.nih.gov/pubmed/29127410
http://dx.doi.org/10.1038/s41598-017-15717-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5681555/
https://www.ncbi.nlm.nih.gov/pubmed/29127410
http://dx.doi.org/10.1038/s41598-017-15717-7

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