Gadd45a opens up the promoter regions of miR-295 facilitating pluripotency induction

MicroRNAs (miRNAs) play crucial roles in the establishment of pluripotent state by controlling pluripotent network. However, the molecular mechanisms controlling miRNAs during somatic cell reprogramming remain obscure. In this study, we show Gadd45a (growth arrest and DNA-damage-inducible protein 45...

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Autores principales: Li, Linpeng, Chen, Keshi, Wu, Yi, Long, Qi, Zhao, Danyun, Ma, Bochao, Pei, Duanqing, Liu, Xingguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682663/
https://www.ncbi.nlm.nih.gov/pubmed/29022923
http://dx.doi.org/10.1038/cddis.2017.497
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author Li, Linpeng
Chen, Keshi
Wu, Yi
Long, Qi
Zhao, Danyun
Ma, Bochao
Pei, Duanqing
Liu, Xingguo
author_facet Li, Linpeng
Chen, Keshi
Wu, Yi
Long, Qi
Zhao, Danyun
Ma, Bochao
Pei, Duanqing
Liu, Xingguo
author_sort Li, Linpeng
collection PubMed
description MicroRNAs (miRNAs) play crucial roles in the establishment of pluripotent state by controlling pluripotent network. However, the molecular mechanisms controlling miRNAs during somatic cell reprogramming remain obscure. In this study, we show Gadd45a (growth arrest and DNA-damage-inducible protein 45a) enhances reprogramming by activating miR-295. Furthermore, we show that Gadd45a binds the promoter regions of miR-295. Nuclease accessibility assay indicates that Gadd45a opens the promoter regions of miR-295. Levels of H3K9Ac and H3K27Ac on the promoter regions of miR-295 were also increased. In conclusion, our results indicate that Gadd45a relaxes the promoter regions of miR-295 and promotes the expression of miR-295 during reprogramming, implying a concise mechanism of Gadd45a and miR-290 cluster cooperation in cell-fate determination.
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spelling pubmed-56826632017-11-16 Gadd45a opens up the promoter regions of miR-295 facilitating pluripotency induction Li, Linpeng Chen, Keshi Wu, Yi Long, Qi Zhao, Danyun Ma, Bochao Pei, Duanqing Liu, Xingguo Cell Death Dis Original Article MicroRNAs (miRNAs) play crucial roles in the establishment of pluripotent state by controlling pluripotent network. However, the molecular mechanisms controlling miRNAs during somatic cell reprogramming remain obscure. In this study, we show Gadd45a (growth arrest and DNA-damage-inducible protein 45a) enhances reprogramming by activating miR-295. Furthermore, we show that Gadd45a binds the promoter regions of miR-295. Nuclease accessibility assay indicates that Gadd45a opens the promoter regions of miR-295. Levels of H3K9Ac and H3K27Ac on the promoter regions of miR-295 were also increased. In conclusion, our results indicate that Gadd45a relaxes the promoter regions of miR-295 and promotes the expression of miR-295 during reprogramming, implying a concise mechanism of Gadd45a and miR-290 cluster cooperation in cell-fate determination. Nature Publishing Group 2017-10 2017-10-12 /pmc/articles/PMC5682663/ /pubmed/29022923 http://dx.doi.org/10.1038/cddis.2017.497 Text en Copyright © 2017 The Author(s) http://creativecommons.org/licenses/by/4.0/ Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Original Article
Li, Linpeng
Chen, Keshi
Wu, Yi
Long, Qi
Zhao, Danyun
Ma, Bochao
Pei, Duanqing
Liu, Xingguo
Gadd45a opens up the promoter regions of miR-295 facilitating pluripotency induction
title Gadd45a opens up the promoter regions of miR-295 facilitating pluripotency induction
title_full Gadd45a opens up the promoter regions of miR-295 facilitating pluripotency induction
title_fullStr Gadd45a opens up the promoter regions of miR-295 facilitating pluripotency induction
title_full_unstemmed Gadd45a opens up the promoter regions of miR-295 facilitating pluripotency induction
title_short Gadd45a opens up the promoter regions of miR-295 facilitating pluripotency induction
title_sort gadd45a opens up the promoter regions of mir-295 facilitating pluripotency induction
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682663/
https://www.ncbi.nlm.nih.gov/pubmed/29022923
http://dx.doi.org/10.1038/cddis.2017.497
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