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The isolation of primary hepatocytes from human tissue: optimising the use of small non-encapsulated liver resection surplus

Two-step perfusion is considered the gold standard method for isolating hepatocytes from human liver tissue. As perfusion may require a large tissue specimen, which is encapsulated and has accessible vessels for cannulation, only a limited number of tissue samples may be suitable. Therefore, the aim...

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Detalles Bibliográficos
Autores principales: Green, Charlotte J., Charlton, Catriona A., Wang, Lai-Mun, Silva, Michael, Morten, Karl J., Hodson, Leanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682876/
https://www.ncbi.nlm.nih.gov/pubmed/28717878
http://dx.doi.org/10.1007/s10561-017-9641-6