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Structure of the staphylococcal enterotoxin B vaccine candidate S19 showing eliminated superantigen activity

Four mutations (N23A, Y90A, R110A and F177A) were introduced into S19, a vaccine candidate for staphylococcal enterotoxin B (SEB), resulting in a lower binding affinity towards the T-cell receptor beta chain (TCB) and reducing its superantigen activity. The structure of S19 was solved and was superp...

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Autores principales: Jeong, Woo Hyeon, Song, Dong Hyun, Hur, Gyeung Haeng, Jeong, Seong Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5683028/
https://www.ncbi.nlm.nih.gov/pubmed/29095152
http://dx.doi.org/10.1107/S2053230X17014844
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author Jeong, Woo Hyeon
Song, Dong Hyun
Hur, Gyeung Haeng
Jeong, Seong Tae
author_facet Jeong, Woo Hyeon
Song, Dong Hyun
Hur, Gyeung Haeng
Jeong, Seong Tae
author_sort Jeong, Woo Hyeon
collection PubMed
description Four mutations (N23A, Y90A, R110A and F177A) were introduced into S19, a vaccine candidate for staphylococcal enterotoxin B (SEB), resulting in a lower binding affinity towards the T-cell receptor beta chain (TCB) and reducing its superantigen activity. The structure of S19 was solved and was superposed on the native or complex structure of SEB. In the superposition model, mutations that were introduced seemed to reduce the number of hydrogen bonds at the SEB–TCB interface. S19 also displayed an unexpected structural change around the flexible-loop region owing to the Y90A mutation. This local structural change provided evidence that the mutated form of S19 could have a lower affinity for major histocompatibility complex (MHC) class II than wild-type SEB.
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spelling pubmed-56830282017-11-17 Structure of the staphylococcal enterotoxin B vaccine candidate S19 showing eliminated superantigen activity Jeong, Woo Hyeon Song, Dong Hyun Hur, Gyeung Haeng Jeong, Seong Tae Acta Crystallogr F Struct Biol Commun Research Communications Four mutations (N23A, Y90A, R110A and F177A) were introduced into S19, a vaccine candidate for staphylococcal enterotoxin B (SEB), resulting in a lower binding affinity towards the T-cell receptor beta chain (TCB) and reducing its superantigen activity. The structure of S19 was solved and was superposed on the native or complex structure of SEB. In the superposition model, mutations that were introduced seemed to reduce the number of hydrogen bonds at the SEB–TCB interface. S19 also displayed an unexpected structural change around the flexible-loop region owing to the Y90A mutation. This local structural change provided evidence that the mutated form of S19 could have a lower affinity for major histocompatibility complex (MHC) class II than wild-type SEB. International Union of Crystallography 2017-10-20 /pmc/articles/PMC5683028/ /pubmed/29095152 http://dx.doi.org/10.1107/S2053230X17014844 Text en © Jeong et al. 2017 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/
spellingShingle Research Communications
Jeong, Woo Hyeon
Song, Dong Hyun
Hur, Gyeung Haeng
Jeong, Seong Tae
Structure of the staphylococcal enterotoxin B vaccine candidate S19 showing eliminated superantigen activity
title Structure of the staphylococcal enterotoxin B vaccine candidate S19 showing eliminated superantigen activity
title_full Structure of the staphylococcal enterotoxin B vaccine candidate S19 showing eliminated superantigen activity
title_fullStr Structure of the staphylococcal enterotoxin B vaccine candidate S19 showing eliminated superantigen activity
title_full_unstemmed Structure of the staphylococcal enterotoxin B vaccine candidate S19 showing eliminated superantigen activity
title_short Structure of the staphylococcal enterotoxin B vaccine candidate S19 showing eliminated superantigen activity
title_sort structure of the staphylococcal enterotoxin b vaccine candidate s19 showing eliminated superantigen activity
topic Research Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5683028/
https://www.ncbi.nlm.nih.gov/pubmed/29095152
http://dx.doi.org/10.1107/S2053230X17014844
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