Disruption of diphthamide synthesis genes and resulting toxin resistance as a robust technology for quantifying and optimizing CRISPR/Cas9-mediated gene editing

We have devised an effective and robust method for the characterization of gene-editing events. The efficacy of editing-mediated mono- and bi-allelic gene inactivation and integration events is quantified based on colony counts. The combination of diphtheria toxin (DT) and puromycin (PM) selection e...

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Detalles Bibliográficos
Autores principales: Killian, Tobias, Dickopf, Steffen, Haas, Alexander K., Kirstenpfad, Claudia, Mayer, Klaus, Brinkmann, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684134/
https://www.ncbi.nlm.nih.gov/pubmed/29133816
http://dx.doi.org/10.1038/s41598-017-15206-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684134/
https://www.ncbi.nlm.nih.gov/pubmed/29133816
http://dx.doi.org/10.1038/s41598-017-15206-x

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