Redox-sensitive GFP fusions for monitoring the catalytic mechanism and inactivation of peroxiredoxins in living cells

Redox-sensitive green fluorescent protein 2 (roGFP2) is a valuable tool for redox measurements in living cells. Here, we demonstrate that roGFP2 can also be used to gain mechanistic insights into redox catalysis in vivo. In vitro enzyme properties such as the rate-limiting reduction of wild type and...

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Detalles Bibliográficos
Autores principales: Staudacher, Verena, Trujillo, Madia, Diederichs, Tim, Dick, Tobias P., Radi, Rafael, Morgan, Bruce, Deponte, Marcel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684490/
https://www.ncbi.nlm.nih.gov/pubmed/29128826
http://dx.doi.org/10.1016/j.redox.2017.10.017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684490/
https://www.ncbi.nlm.nih.gov/pubmed/29128826
http://dx.doi.org/10.1016/j.redox.2017.10.017

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