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SUMOylation regulates the localization and activity of Polo-like kinase 1 during cell cycle in the silkworm, Bombyx mori

Polo-like kinase 1 (Plk1) is a crucial cell cycle regulator by its specific localization and activity during cell cycle. It has been shown that the phosphorylation and ubiquitylation of Plk1 are required for its own activation and localization. Here, we report that SUMOylation regulates the activity...

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Autores principales: Li, Zhiqing, Cui, Qixin, Xu, Jian, Cheng, Daojun, Wang, Xiaoyan, Li, Bingqian, Lee, Jae Man, Xia, Qingyou, Kusakabe, Takahiro, Zhao, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686133/
https://www.ncbi.nlm.nih.gov/pubmed/29138491
http://dx.doi.org/10.1038/s41598-017-15884-7
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author Li, Zhiqing
Cui, Qixin
Xu, Jian
Cheng, Daojun
Wang, Xiaoyan
Li, Bingqian
Lee, Jae Man
Xia, Qingyou
Kusakabe, Takahiro
Zhao, Ping
author_facet Li, Zhiqing
Cui, Qixin
Xu, Jian
Cheng, Daojun
Wang, Xiaoyan
Li, Bingqian
Lee, Jae Man
Xia, Qingyou
Kusakabe, Takahiro
Zhao, Ping
author_sort Li, Zhiqing
collection PubMed
description Polo-like kinase 1 (Plk1) is a crucial cell cycle regulator by its specific localization and activity during cell cycle. It has been shown that the phosphorylation and ubiquitylation of Plk1 are required for its own activation and localization. Here, we report that SUMOylation regulates the activity of Plk1 in the lepidopteran insect of Bombyx mori. In the absence of SUMOylation, it causes the lost localization of Plk1 on centrosomes and kinetochores, as well as an uneven distribution in midzone. We further identify that the putative SUMOylation site of Bombyx Plk1 at lysine 466 is required for its localization on centrosomes, and K466 mutation in Plk1 could influence its interaction with Smt3/Ubc9 complex. These findings are also confirmed by Drosophila Polo and human Plk1, which together reveals a conserved role of Plk1 SUMOylation in mammals. Moreover, conjugation of Smt3 to Plk1 SUMOylation mutant promotes its localization on centrosomes and kinetochores, and rescues functional defects of chromosome alignment in cells depleted of endogenous Plk1. Altogether, the present data indicate that the SUMOylation of Plk1 could participate in proper chromosome alignment and segregation during mitosis, and provides a novel layer for the regulation of Plk1 localization and activity throughout cell cycle.
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spelling pubmed-56861332017-11-21 SUMOylation regulates the localization and activity of Polo-like kinase 1 during cell cycle in the silkworm, Bombyx mori Li, Zhiqing Cui, Qixin Xu, Jian Cheng, Daojun Wang, Xiaoyan Li, Bingqian Lee, Jae Man Xia, Qingyou Kusakabe, Takahiro Zhao, Ping Sci Rep Article Polo-like kinase 1 (Plk1) is a crucial cell cycle regulator by its specific localization and activity during cell cycle. It has been shown that the phosphorylation and ubiquitylation of Plk1 are required for its own activation and localization. Here, we report that SUMOylation regulates the activity of Plk1 in the lepidopteran insect of Bombyx mori. In the absence of SUMOylation, it causes the lost localization of Plk1 on centrosomes and kinetochores, as well as an uneven distribution in midzone. We further identify that the putative SUMOylation site of Bombyx Plk1 at lysine 466 is required for its localization on centrosomes, and K466 mutation in Plk1 could influence its interaction with Smt3/Ubc9 complex. These findings are also confirmed by Drosophila Polo and human Plk1, which together reveals a conserved role of Plk1 SUMOylation in mammals. Moreover, conjugation of Smt3 to Plk1 SUMOylation mutant promotes its localization on centrosomes and kinetochores, and rescues functional defects of chromosome alignment in cells depleted of endogenous Plk1. Altogether, the present data indicate that the SUMOylation of Plk1 could participate in proper chromosome alignment and segregation during mitosis, and provides a novel layer for the regulation of Plk1 localization and activity throughout cell cycle. Nature Publishing Group UK 2017-11-14 /pmc/articles/PMC5686133/ /pubmed/29138491 http://dx.doi.org/10.1038/s41598-017-15884-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Zhiqing
Cui, Qixin
Xu, Jian
Cheng, Daojun
Wang, Xiaoyan
Li, Bingqian
Lee, Jae Man
Xia, Qingyou
Kusakabe, Takahiro
Zhao, Ping
SUMOylation regulates the localization and activity of Polo-like kinase 1 during cell cycle in the silkworm, Bombyx mori
title SUMOylation regulates the localization and activity of Polo-like kinase 1 during cell cycle in the silkworm, Bombyx mori
title_full SUMOylation regulates the localization and activity of Polo-like kinase 1 during cell cycle in the silkworm, Bombyx mori
title_fullStr SUMOylation regulates the localization and activity of Polo-like kinase 1 during cell cycle in the silkworm, Bombyx mori
title_full_unstemmed SUMOylation regulates the localization and activity of Polo-like kinase 1 during cell cycle in the silkworm, Bombyx mori
title_short SUMOylation regulates the localization and activity of Polo-like kinase 1 during cell cycle in the silkworm, Bombyx mori
title_sort sumoylation regulates the localization and activity of polo-like kinase 1 during cell cycle in the silkworm, bombyx mori
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686133/
https://www.ncbi.nlm.nih.gov/pubmed/29138491
http://dx.doi.org/10.1038/s41598-017-15884-7
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