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AFEAP cloning: a precise and efficient method for large DNA sequence assembly

BACKGROUND: Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembly method named AFEAP cloning (Assembly of Fragment Ends After PCR), which allow...

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Detalles Bibliográficos
Autores principales: Zeng, Fanli, Zang, Jinping, Zhang, Suhua, Hao, Zhimin, Dong, Jingao, Lin, Yibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686892/
https://www.ncbi.nlm.nih.gov/pubmed/29137618
http://dx.doi.org/10.1186/s12896-017-0394-x